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Man cells using an ERV-3 real time PCR assay. J Virol Methods 2001, 91:109?17. 26. Lu X, Holloway B, Dare RK, Kuypers J, Yagi S, Williams JV, Hall CB, Erdman DD: Real-time reverse transcription-PCR assay for comprehensive detection of human rhinoviruses. J clin microbiol 2008, 46:533?. 27. Whiley DM, Sloots TP: A 5-nuclease real-time reverse transcriptasepolymerase chain reaction assay for the det
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Ncytial virus RNA. Arch Dis Child 2007, 92:1046?. 20. O'Grady K-AF, Torzillo PJ, Rockett RJ, Whiley DM, Nissen MD, Sloots TP, Lambert SB: Successful application of a simple specimen transport method for the conduct of respiratory virus surveillance in remote Indigenous communities in Australia. Trop Med Int Health 2011, 16:766?2. 21. Akmatov MK, Krebs S, Preusse M, Gatzemeier A, Frischmann U, Schu
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Atory syncytial virus infection in immunocompromised adults. J clin microbiol 2003, 41:4378?1. 29. Lambert SB, Allen KM, Druce JD, Birch CJ, Mackay IM, Carlin JB, Carapetis JR, Sloots TP, Nissen MD, Nolan TM: Community epidemiology of human metapneumovirus, human coronavirus NL63, and other respiratory30.31.32.33.34.35.36. 37. 38.viruses in healthy preschool-aged children using parent-collected sp
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Crobiol 2009, 47:2981?. Van Elden LJR, Van Loon AM, Van Alphen F, Hendriksen KAW, Hoepelman AIM, Van Kraaij MGJ, Oosterheert J-J, Schipper P, Schuurman R, Nijhuis M: Frequent detection of human coronaviruses in clinical specimens from patients with respiratory tract infection by use of a novel real-time reverse-transcriptase polymerase chain reaction. J Infect Dis 2004, 189:652?. Dare RK, Fry AM,
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Crobiol 2009, 47:2981?. Van Elden LJR, Van Loon AM, Van Alphen F, Hendriksen KAW, Hoepelman AIM, Van Kraaij MGJ, Oosterheert J-J, Schipper P, Schuurman R, Nijhuis M: Frequent detection of human coronaviruses in clinical specimens from patients with respiratory tract infection by use of a novel real-time reverse-transcriptase polymerase chain reaction. J Infect Dis 2004, 189:652?. Dare RK, Fry AM,
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En implicated in many persistent and chronic diseases such as cystic fibrosis, endocarditis, and infections caused by biofilms growing on incorporated foreign materials, e.g. stents, indwelling catheters, bone implants, and artificial valves [1-5]. Dental caries and periodontal diseases, which are among the most common bacterial infections in humans, are caused by biofilms known as dental plaque t
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Ory virus detection. Indeed, this may have broader implications since nasal swabs are beginning to replace the traditional, but more invasive nasopharyngeal swab or aspirate sampling techniques in hospitals and clinics, especially following the 2009 influenza pandemic [17]. Thus, similar ERV3 testing strategies could be used by diagnostic laboratories to improve or monitor sample collection qualit
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Asopharyngeal aspirate using real-time polymerase chain reaction. Eur J Clin Microbiol Infect Dis 2010, 29:365?1. 17. Lambert SB, Allen KM, Nolan TM: Parent-collected respiratory specimens novel method for respiratory virus and vaccine efficacy research. Vaccine 2008, 26:1826?1. 18. Lambert SB, Whiley DM, O'Neill NT, Andrews EC, Canavan FM, Bletchly C, Siebert DJ, Sloots TP, Nissen MD: Comparing