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S, but in fact appeared to increase with time taken to reach the laboratory. While the observed increase was unexpected and may have occurred simply by chance, it is plausible that viral nucleic acids were protected to some extent by being encapsulated within the viral capsid, and by using viral transport medium in the swabs. Fungi were found to be associated with both reduced ERV3 detection and,
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Man cells using an ERV-3 real time PCR assay. J Virol Methods 2001, 91:109?17. 26. Lu X, Holloway B, Dare RK, Kuypers J, Yagi S, Williams JV, Hall CB, Erdman DD: Real-time reverse transcription-PCR assay for comprehensive detection of human rhinoviruses. J clin microbiol 2008, 46:533?. 27. Whiley DM, Sloots TP: A 5-nuclease real-time reverse transcriptasepolymerase chain reaction assay for the det
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En implicated in many persistent and chronic diseases such as cystic fibrosis, endocarditis, and infections caused by biofilms growing on incorporated foreign materials, e.g. stents, indwelling catheters, bone implants, and artificial valves [1-5]. Dental caries and periodontal diseases, which are among the most common bacterial infections in humans, are caused by biofilms known as dental plaque t
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En implicated in many persistent and chronic diseases such as cystic fibrosis, endocarditis, and infections caused by biofilms growing on incorporated foreign materials, e.g. stents, indwelling catheters, bone implants, and artificial valves [1-5]. Dental caries and periodontal diseases, which are among the most common bacterial infections in humans, are caused by biofilms known as dental plaque t
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Oots TP: Detection of human bocavirus in respiratory, fecal, and blood samples by real-time PCR. J Med Virol 2009, 493(November 2008):488?93. Dang X, Bialasiewicz S, Nissen MD, Sloots TP, Koralnik IJ, Tan CS: Infrequent detection of KI. WU and MC polyomaviruses in immunosuppressed individuals with or without progressive multifocal leukoencephalopathy. PloS One 2011, 6:e16736. Pryce TM, Palladino S
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Man cells using an ERV-3 real time PCR assay. J Virol Methods 2001, 91:109?17. 26. Lu X, Holloway B, Dare RK, Kuypers J, Yagi S, Williams JV, Hall CB, Erdman DD: Real-time reverse transcription-PCR assay for comprehensive detection of human rhinoviruses. J clin microbiol 2008, 46:533?. 27. Whiley DM, Sloots TP: A 5-nuclease real-time reverse transcriptasepolymerase chain reaction assay for the det
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Stralia. 6Microbiology Division, Pathology Queensland Central Laboratory, Brisbane Queensland 4006, Australia.Received: 24 June 2013 Accepted: 20 December 2013 Published: 9 January 2014 References 1. Monto AS: Epidemiology of viral respiratory infections. Am J Med 2002, 112:4S?2S. 2. Beck ET, Henrickson KJ: Molecular diagnosis of respiratory viruses. Future Microbiol 2010, 5:901?16. 3. Calvo C, Po
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Ncytial virus RNA. Arch Dis Child 2007, 92:1046?. 20. O'Grady K-AF, Torzillo PJ, Rockett RJ, Whiley DM, Nissen MD, Sloots TP, Lambert SB: Successful application of a simple specimen transport method for the conduct of respiratory virus surveillance in remote Indigenous communities in Australia. Trop Med Int Health 2011, 16:766?2. 21. Akmatov MK, Krebs S, Preusse M, Gatzemeier A, Frischmann U, Schu