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Was especially evident during the warm, humid spring and summer months, which leads us to speculate that fungal contamination occurred during sample collection and was influenced by the aforementioned environmental factors. Unfortunately, we could not explore this further as it was beyond the scope of the present study. In addition, while mould growth proved to be an issue in the subtropical clima
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And The Children's Health Foundation Queensland. Ms Alsaleh received a scholarship from the Saudi Arabia Ministry of Higher Education- King Saud University. Author details Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia. 2Queensland Paediatric Infectious Disease Laboratory, Royal Children's Hospital, Brisbane Queensland 4029, Aust
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Y arrival should be monitored and feedback provided if delays occur. Finally, if delays are expected swabs should be placed in the household refrigerator until mailed to the laboratory [20].Abbreviations ARI: Acute respiratory infection; Ct: Cycle threshold; DNA: Deoxyribonucleic acid; EHV1: Equine herpes virus 1; ERV3: Endogenous retrovirus 3; ITS: Internal transcribed spacer; OR: Odds ratio; ORC
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Issen MD, Grimwood K: Observational Research in Childhood Infectious Diseases (ORChID): a dynamic birth cohort study. BMJ open 2012, 2:002134 2012. 23. Australia Post: Dangerous and prohibited goods and packaging-post guide. 2009. http://auspost.com.au/media/documents/dangerous-prohibitedgoods-packaging-post-guide.pdf. 24. Bialasiewicz S, Whiley DM, Buhrer-Skinner M, Bautista C, Barker K, Aitken S
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This shows the importance of measuring human DNA as a marker for epithelial cells in swab samples, which if tested and monitored in real time during the study, can identify problems associated with collection that can be addressed quickly. This is illustrated in the current study when a sudden increase in ERV3 negative samples was observed. Parents were contacted and reminded about sample collecti
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And The Children's Health Foundation Queensland. Ms Alsaleh received a scholarship from the Saudi Arabia Ministry of Higher Education- King Saud University. Author details Queensland Children's Medical Research Institute, The University of Queensland, Brisbane Queensland 4029, Australia. 2Queensland Paediatric Infectious Disease Laboratory, Royal Children's Hospital, Brisbane Queensland 4029, Aust
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Man cells using an ERV-3 real time PCR assay. J Virol Methods 2001, 91:109?17. 26. Lu X, Holloway B, Dare RK, Kuypers J, Yagi S, Williams JV, Hall CB, Erdman DD: Real-time reverse transcription-PCR assay for comprehensive detection of human rhinoviruses. J clin microbiol 2008, 46:533?. 27. Whiley DM, Sloots TP: A 5-nuclease real-time reverse transcriptasepolymerase chain reaction assay for the det
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Ormed the experiments: AA, DW, SB. Analyzed the data: RW, AA, SL. Contributed reagents/materials/analysis tools: AA, DW, SB, SL, TS, KG. Wrote the manuscript: AA, DW, SL, KG. Approved the final manuscript: AA, DW, SB, SL, RW, MN, TS, KG. All authors read and approved the final manuscript. Acknowledgements We thank the ORChID study team: Anne Cook, Hannah Cox, Jane Gaydon, Rebecca Holding, Kevin Ja