Calization of CRH and p-NR1 in the PVN. A-C: Sections from EA-treated naive rats were double-labeled with anti-CRH (red) and antip-NR1 (green). A: CRH-immunoreactive neurons in the PVN. B: p-NRI-immunoreactive neurons in the PVN. C: Merged graphs of A and B. Small arrows indicate examples of double-labeled CRH/p-NR1 neuron (yellow); Arrowheads point to single-labeled CRH and p-NR1. The insets in A
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